Figure 1. LIN-37 and LIN-54 are expressed broadly in nuclei throughout development. (A) Anti-LIN-37 antibodies were used to blot extracts from WT and
lin-37(
n758) mutant animals. The asterisk denotes nonspecific immunoreactivity. (B) Anti-LIN-54 antibodies were used to blot extracts from both WT and
lin-54(
n3423) mutant animals, which were derived from heterozygous mothers. (C and D) Whole-mount staining with anti-LIN-37 antisera of an embryo and the midbody of an L1 larva, respectively. (E and F) DAPI staining of the animals shown in C and D. (G and H) Whole-mount staining with anti-LIN-54 antisera of an embryo and the midbody of an adult hermaphrodite, showing the germ line, respectively. (J and K) DAPI staining of the animals shown in G and H. (I and L) Enlargements of the boxed portions of H and K, respectively. (Scale bars: 10 um.)