Figure 3.
nas-37 is transcribed in the hypoderm. A
nas-37 promoter GFP transcriptional fusion (Pnas-37::GFP) 3807 bp of upstream promoter was fused to the GFP open reading frame at the start codon of
nas-37. In these confocal micrographs, anterior is left and dorsal is up. (A) An L2 larva exhibits strong expression of the reporter construct in the hypodermis. Intense staining on the dorsal and ventral surfaces represents the hypodermal ridges; expression is not observed in the nervous system or in the intestine. Seam cells do not express
nas-37. (B) The posterior region of the same animal shows strong expression in the rectal epithelial (REP) cells and the hypodermis. (C) An anterior view of an L4 larva shows hypodermal expression and strong expression in the lateral seam cells. (D) An anterior view of an early adult worm shows dim expression in the arcade cells, lateral seam cells and Hyp5. (E) Pnas-37::GFP strongly expresses in the vulval epithelial cells throughout the adult stage, and (F) in the rectal epithelial cells, which do not secrete a cuticle, but is not expressed in the adjacent hypodermal cells, which secrete the cuticle of the rectum (arrow).