Figure 2. GFP::POP-1 localization in the Z1/Z4 daughters. All panels show L1 larvae expressing GFP::POP-1
(d1-5) [animals expressing GFP::POP-1(FL) show similar localization]. The gonad is indicated by the dashed circle. Nuclei of sister cells are indicated by brackets. Images alongside each other are of the same animal: DIC on the left and fluorescence on the right. Bars, 5 um. (A and B) The T daughters are posterior; (C-J) anterior is to the left. (A and B) In the V and T cell daughters, GFP::POP-1 shows more GFP in nuclei of anterior sister cells than in posterior sister cells. (C-F) GFP:: POP-1 is higher in nuclei of the proximal Z1/Z4 daughters than in the distal daughters. GFP-positive puncta are present in the nuclei of the proximal daughters (D, arrowheads). (G-J)
lit-1(RNAi) or
wrm-1(RNAi) results in similar nuclear levels of GFP::POP-1 in Z1/Z4 daughters. GFP-positive puncta are often seen in both Z1/Z4 daughters (H, arrowheads). The Z1 daughters are shown.