Figure 4. Expression and rescuing activity of VPS-18. (A) VPS-18::GFP expression in embryo (a and b), larva (c and d), adult coelomocyte (e and f), and germline (g and h) as well as the dissected-out germline (i and j). Arrows in (h and j) indicate germline sheath cells. The scale bars represent 20 um (a-d, i, and j), 5 um (e and f), and 10 um (g and h). (B) Rescuing activity of Pvps-18vps-18::gfp, Pvps-18
vps-18(C859A, H861A)::gfp, Pced-1vps-18 and Pegl-1vps-18 in
vps-18(
tm1125) embryos. Embryonic cell corpses from 15 animals were scored for comma- (gray bar) and twofold stage (black bar). Comparisons were performed between
vps-18(
tm1125) and N2 animals and between
vps-18(
tm1125) and transgenic animals by using unpaired t test. For transgenes, only one transgenic line from a total of three lines that exhibit similar rescuing activity was shown. (C) Rescuing activity of Pvps-18vps-18::gfp, Pvps-18
vps-18(C859A, H861A)::gfp, Pvps-33mVps-18, and Plim-7vps-18::gfp in
vps-18(
tm1125) germline. Germ cell corpses in one gonad arm of 15 animals were scored 48 h post-L4 stage for two to three independent transgenic lines as indicated by black, waved, and gray columns. Comparisons were performed between
vps-18(
tm1125) and all transgenic lines by using unpaired t test. Error bars represent SEM, and double asterisks (**) indicate p < 0.001.