Figure 5. Localization of a SRF-3::GFP and a SRF-3::NLS::lacZ fusion. For each GFP, the corresponding DIC is shown below. Unless otherwise indicated, the scale bar represents 10 μm. A, seam cell expression in late embryo prior to hatching. B, seam cell expression in L2 larva. C, intracellular SRF-3::GFP localization showing a perinuclear staining. Photograph is taken from a seam cell of an L2 larva. D,
g1/g2 expression at higher magnification showing strong fluorescence in the cell bodies and a very weak fluorescence of processes (white arrows). E, spermatheca expression at higher magnification. F, β-galactosidase staining of an L4 larvae expressing
srf-3::lacZ. Visible are the seam cells that start to fuse and the glandular cells (arrow). G, β-galactosidase staining of the glandular cells
g1 and
g2 (black arrows) in a L1 larvae.