Figure 1. Poly-ubiquitin chains labelling on sperm derived organelles in early C elegans embryos:(A) Scheme of the Tandem-repeated Ubiquitin-Binding Entities (TUBEs) fused to fluorescein used to localize and visualize poly-ubiquitin chains. TUBEs are tetramerized ubiquitin association (UBA) domains with flexible poly-glycine links which bind poly-ubiquitin chains on substrates. Early C. elegans embryos labelled K48-TUBEs (B) or K63-TUBEs (C) (Green) showed a strong poly-ubiquitylation on MOs (red) for both K48- and K63-poly-ubiquitin chains. (D) In contrast, no poly-ubiquitin signal with Pan-TUBEs probes (green) was observed on sperm derived mitochondria (red) in early C. elegans embryos. The green rings observed are most likely poly-ubiquitylated MOs. Scale bars represent 5 µm in full embryo images and 2 µm in inserts.
x2640;︎and
x2642; signs on images mark the presence of the respective pronuclei. Spz indicates spermatozoa outside the embryo. All images are maximum intensity projection images.