Figure 5. Localization of UNC-112::GFP fluorescence and MH25 (PAT-3/beta-integrin) immunofluorescence in adults and embryos. (A) UNC-112::GFP fluorescence in adult body wall muscle of a rescued
unc-112(
st581); raEx16[
unc-112::GFP;
rol-6(
su1006)] hermaphrodite. (B-D) colocalization of UNC-112::GFP (green) with PAT-3/integrin (red) in the same body wall muscle cell of a +/+; raEx16[
unc-112::GFP;
rol-6(
su1006)] hermaphrodite; (B) UNC-112::GFP fluorescence, (C) MH25 immunofluorescence, (D) both GFP and MH25. Arrowheads indicate the M-line; small arrows indicate dense bodies; and large arrows indicate adhesion plaques between muscle cells. (E-G) colocalization of UNC-112::GFP (green) with PAT-3/integrin (red) in the body wall muscle of a 1.5-fold +/+; raEx16[
unc-112::GFP;
rol-6(
su1006)] embryo. (E) UNC-112::GFP fluorescence, (F) MH25 immunofluorescence, (G) both GFP and MH25. All images are projected to show a single muscle quadrant. Bar, 10 um.