Figure 6. MUA-3 colocalizes with cytoplasmic IFs at hypodermal hemidesmosomes. It is also associated with MUA-3 at other sites of MUA-3 localization in animals double stained with anti-p70
(IF) and MUA-3 antibodies. (A) IFs associated with hypodermal hemidesmosomes visualized with anti-p70
in an adult. (B) Same animal stained for MUA-3. (C) Merge of A and B. (D) p70
localization in threefold embryo. Embryo is folded on itself: t indicates location of tail; the dashed line shows location of fold between posterior and central body regions, the anterior region is folded under the posterior end and not in field of view. Ventral- and dorsal-staining stripes in hypodermis are visible and indicated by arrowheads and an arrow, respectively. (E) Same embryo showing MUA-3 localization. Note stripe pattern similar to p70
, indicated by arrowheads and an arrow. (F) Merge of E and F. (G) Duct and pore cells showing p70
IF localization. Ventral is down, dashed line indicates location of body wall (not visible), arrows indicate pore cell (note lack of staining in hollow lumen), and the arrowhead indicates the duct cell. (H) Same, showing MUA-3 localization. Note that MUA-3 is located closer to the lumenal cuticle and extends closer to body wall than the p70
, especially apparent in the pore cell (arrows). Arrowhead indicates duct cell. (I) Merge of G and H. Bars, 10 um.