Figure 2. a, OSM-10 is expressed in ASH, ASI, PHA, and PHB. Expression was monitored by staining fixed animals in whole-mount preparations with a polyclonal rabbit anti-OSM-10 antibody. Cells were identified on the basis of their nuclear positions and axon morphologies (Sulston et al., 1983; White et al., 1986). The OSM-10 protein is diffusely distributed throughout the cell body, sensory processes, cilium, and axons of expressing cells. The cellular and subcellular expression pattern of the OSM-10:: GFP reporter construct nuIs11I is identical. Cells in gray are located on the right side of the animal; cells in black are on the left. Scale bars, 25 um. b, Polyclonal rabbit anti-OSM-10 antisera recognize a 49 kDa band on Western blots from extracts of wild-type,
osm-10(
n1602),
osm-3(
p802), and nuIs11(pKP#58, pJM#24) animals. An additional band at ~60 kDa corresponding to the OSM-10:: GFP fusion protein is visible in the nuIs11I lane. The same blot probed with anti-tubulin antibodies (N356; Amersham) is shown at the bottom. c, OSM-10 immunoreactivity in ASH neurons (visualized as in a) is shown. OSM-10 immunoreactivity is excluded from the nucleus.Left, Deconvoluted images of an ASH cell body are shown. Middle,Right, OSM-10 immunoreactivity is severely reduced in
osm-10(
nr2076) animals but is found at wild-type levels in
eos-1(
nu288) or
eos-2(
nu268) (data not shown) animals.