Figure 3.
psa-3 Expression Is Regulated by
pop-1,
nob-1, and
ceh-20. (A-C) The expression of
psa-3::gfp in the (A) T cell, (B) its daughters, and (C) its granddaughters in wild-type. Arrowheads indicate the nucleus of the T cell and its descendants. Anterior is to the left, and ventral is to the bottom.(D-G)
psa-3 expression in the granddaughters of the T cell was examined, by using either (D) mut-
psa-3::gfp with an altered POP-1 binding site in wild-type or (E)
psa-3::gfp in
pop-1(
q645), (F)
nob-1(
os6), or (G)
ceh-20(
os39) mutants.(H-J) The intensity of
psa-3::gfp was examined in the T cell or its progeny. In each experiment, more than 20 samples were scored. 'Early' and 'late' indicate before and after the V6 cell division, respectively, which occurs 30 min after the T cell division. The signal intensity representing 'strong expression' (black bars) or 'weak expression' (gray bars) was more or less than five times higher than background (no signal, open bars), respectively. (H)
psa-3 expression in T cells, daughter cells, or granddaughter cells in wild-type. (I) Expression of the indicated
psa-3::gfp fusion constructs (see Figure 4A) in the posterior granddaughter cells (T.pa or T.pp) was examined in wild-type (WT), or
nob-1(
os6),
ceh-20(
os39), or
pop-1(
q645) mutants. (J) Animals that showed asymmetric or symmetric
psa-3 expression in the daughter cells were scored. 'Asymmetric' (black bars) and 'symmetric' (vertical hatched bars) mean that the intensities were higher in T.p than in T.a and equal between T.a and T.p, respectively.