Figure 2.
lsy-6 Is the First Known Asymmetrically Expressed Component of the Loop in the ASE Neurons (A) Schematics of fosmid reporter genes. (B) Representative YFP expression in animals carrying the
lsy-6::yfp fosmid reporter. Expression is first seen around the bean stage, exclusively in ASEL, and continues in ASEL until adulthood. Animals and ASEL are outlined. For quantification, see Figure S2A. * marks autofluorescence from intestinal cells. (C) Expression of fosmid-based
cog-1::yfp and
die-1::yfp reporters. Both transcription factors start being expressed in ASE only at the 3-fold stage in an asymmetric manner:
cog-1::yfp in ASER and
die-1::yfp in ASEL. A bilaterally expressed
che-1prom::mCherry reporter was used to label the two ASEs. (D) Summary of developmental expression of
lsy-6,
die-1, and
cog-1 in the ASE neurons.