Picture from Harfe BD et al. (1998) Development "Muscle and nerve-specific regulation of a novel NK-2 class homeodomain factor in ...."

Figure 3. Micrographs showing ceh-24 reporter expression and RNA localization. Expression patterns for all constructs were assayed both in transgenic lines and in the first generation following microinjection. Identical expression patterns were observed for all constructs in both heritable lines and F1 assays. (A) ceh-24::lacZ expression in an adult animal. Expression is seen in the eight vulval muscles, eight ventral neurons in the head, and in the most posterior pharyngeal muscle, m8. (B) ceh-24::lacZ expression in the eight vulval muscles of an adult animal. (C) ceh-24::lacZ expression in the adult head. Activity is seen in pharyngeal muscle m8 and in 8-10 ventral head neurons. (D) The ceh-24 vulval muscle enhancer driving lacZ expression from a truncated pes-10 promoter in the eight vulval muscles. (E) The ceh-24 m8 enhancer driving lacZ expression from a truncated myo-2 promoter in the pharyngeal muscle m8. (F-H) In situ localization of ceh-24 RNA. Antisense probes give a pattern with ventral head neurons showing strong expression in comma stage (F), two-fold (G), and three-fold (H) embryos. No signal was seen with a comparable sense' probe (not shown). (I-L) GFP expression driven by concatamerized NdE-boxes. GFP activity is both nuclear and cytoplasmic because of the tendency of the small GFP protein to leak out of the nucleus. (I) Anal depressor muscle (black arrowhead) and the two intestinal muscles (white arrowheads) are shown. Fibers of the intestinal muscles can be seen. (J,K) Higher magnification shows cellular outline of a GFP-positive anal depressor muscle. (L) NdE-box driven GFP expression is seen in vulval and uterine muscles. Scale: Adults are 1 µm long and approximately 100 µm wide. Embryos are 50 µm long.

Picture from Fujita M et al. (1998) Biochem Biophys Res Commun "Expression of spliceosome-associated protein 49 is required for early ...."

FIG. 2. LacZ expression pattern in F1 transformants containing the SAP 49 gene fused with the LacZ gene. (A) LacZ expression in an adult worm was viewed with Nomarski optics. A compilation of the positions of LacZ-positive cells (dots) determined by observation of F1 transformants (n=41) is schematically shown below. (B) Enlarged view of the pharyngeal region of a transformant. The pharyngeal muscle cells are indicated (m3-m8). (C) Enlarged view of the posterior region of a transformant.

Picture from Sakube Y et al. (1997) J Mol Biol "An abnormal ketamine response in mutants defective in the ryanodine receptor ...."

Figure 6. Expression patterns of ryr-1/lacZ transgenes. A and B, Body-wall and pharyngeal muscle nuclei stain in a young adult and a third stage (L3) larva expressing pRRZ4824L. The posterior cells of the pharynx (m5-m8) stain most strongly. Arrowhead, pharyngeal muscles; arrow, vulval muscles; double arrow, anus muscles. C, Typical staining of non-muscle cells (neuronal, intestinal and hypodermal cells) in an L2 larva expressing pRRZ462. Plasmids pRRZ1470, pRRZ1271, pRRZ836 and pRRZ239 produce almost the same pattern but pRRZ1470L stain pharyngeal muscles and non-muscle cells. Bars represent 100 um.

Picture from Anyanful A et al. (2001) J Mol Biol "The third and fourth tropomyosin isoforms of Caenorhabditis elegans are ...."

Figure 3. Tissue expression pattern of CeTMIV isoform with tmy-1::lacZ and tmy-1::gfp transgenes. (a) b-galactosidase expression induced in pharyngeal muscles and intestinal cells of L3 worm by pTMIVZ1957. (b) Strong b-galactosidase activity was observed in m1-m8 muscles, a marginal cell (mc), an epithelial cell (e1) and PIV of the pharynx. (c) Expression in the 20 nuclei of intestinal cells illustrating the characteristic binucleate cells. (d) Expression is first induced in embryos between the 300-cell stage and the comma stage in tissues corresponding to the pharynx and intestines and (e) the pretzel stage expression, enabling identification of the pharyngeal muscles. (f) Expression in the male pharynx was similar to the hermaphrodite, however intestinal expression was restricted more to the posterior end of the digestive canal. Corresponding GFP expressions in pharynx (g) and intestines (h) pTMIVG4349. The b-galactosidase expressions were restricted to the nuclei because the lacZ expression vector contained the SV40 nuclear localization sequence (NLS). This NLS was absent in the gfp expression vector.

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This gene has two distinct modes of expression. The earlier component, shown here, is in a subset of cells of the pharynx, and is nuclear localised. Expression is from late embryogenesis onwards, the subset consisting of the m4 muscles in the mid metacorpus,the m2 muscles and e1 and e2 epithelial cells of the procorpus, and the m8 muscle of the posterior terminal bulb. The second component is in the vm2 vulval muscles. This expression is cytoplasmically localised, and covers only the period around the L4 to adult molt