Figure S13. Expression of
slr-2::GFP. DIC (A, C, E) and corresponding GFP (B, D, F) micrographs of an L4 larvae (A, B), a pre-morphogenetic embryo (~300 cell stage; C, D), and late-stage embryo (E, F). Although brightest in the intestinal posterior, reporter expression was observed throughout the intestine in L4 stage larvae. In addition, L4 larvae exhibited expression in marginal cells and the
m3VR, mC, I5,
m5,
m6, and
m7 cells of the pharynx, as well as a small subset of head neurons and the excretory duct cell (A, B). Ubiquitous expression was observed in the early embryo (C, D), which becomes largely restricted to the intestine by the pretzel-stage, where expression is brightest in the posterior gut region (E, F). In panels A and B, the posterior region displaying the greatest intense intestinal fluorescence is delineated by a solid white brace and the adjacent dimmer region is delineated by a white, dashed brace. The foregut is delineated by a white dotted dashed brace. In panels E and F, the posterior intestinal region is circled. Scale bar: A, B 100 um, C-F, 10 um.