Figure 3.
nsy-4 Is Expressed in AWC Neurons and Epithelial Cells(A) Confocal projection of L1 animal expressing
nsy-4::GFP (GFP driven by 7.7 kb of sequence upstream of
nsy-4). White arrow indicates excretory cell. Yellow arrowheads indicate epidermal cells. (B) Colocalization of
nsy-4::GFP and
odr-3::
lin-10::dsRedII in late L1/early L2 animal. The
odr-3 promoter drives expression primarily in AWC. LIN-10 accumulates in perinuclear intracellular compartments and in axon puncta. (C) Schematic of (B), indicating colocalization of diffuse GFP and punctate LIN-10::dsRed in AWC. (D) Expression of
odr-3::
nsy-4::GFP in a rescued
nsy-4(
ky616) L4 animal. Note nuclear exclusion of GFP in AWC cell body and expression in dendrite, cilium, and axon. (E and F)
str2::GFP in wild-type animals overexpressing
nsy-4 from the
odr-3::
nsy-4 transgene (E) wild-type 1-AWCON phenotype (F) 2-AWCON phenotype. Scale bars, 25 um.