S2 Fig.
let-653 is expressed in external epithelia.Additional data related to Fig 5. (A, B)
let-653pro::GFP reporter sEx10642 expression in the major hypodermis (hyp) at ventral enclosure (A) and in the excretory duct and pore at the 3-fold stage (B). Expression is only occasionally and transiently observed in the canalcell. A' and B' show DIC images for comparison. (E) LET-653b::SfGFP driven by the canal-specific
glt-3 promoter occasionally accumulates in the duct lumen of embryos. (F) LET-653b::SfGFP driven by the non-cycling
lin-48 promoter also shows an oscillatory pattern of accumulation in the duct lumen. n>15 animals for each timepoint. (G-L) Both
let-653pro::LET-653::SfGFP and
let-653pro::ssSfGFP::LET-653 reveal apical secretion and accumulation in extracellular and luminal regions. (G, J) ventral enclosure embryos.(H, K) 3-fold embryos. E',G',H',I',J' show merged DIC images for comparison. (I, L) Both LET-653::SfGFP and ssSfGFP::LET-653 are absent from the early L1 duct cell lumen, after mature cuticle secretion and hatch. K' and L' show absent SfGFP signal. ssSfGFP::LET-653 appears within the space between the old and new cuticles during the L1/L2 molt (M,N). M' and N' show DIC images for comparison. Scale bars, 10 um.