Figure 6.
kpc-1 Is Expressed Broadly but Acts Cell Autonomously to Regulate IL2Q Dauer-Specific Remodeling (A) Ventral z projection of dauer expressing Pkpc-1::GFP with expression in numerous neuronal and nonneuronal cells throughout the head. The scale bar represents 10 um. (B) Ventral body-wall plane of same animal as in (A) showing GFP expression in IL2Q dauer-specific branches (arrow) as well as several additional neuronal commissures (arrowhead, amphid commisure). The scale bar represents 10 mm. (C) Dauer expressing both the IL2-specific reporter Pklp-6::tdTomato (left) and Pkpc-1::GFP (middle). An overlay image (right) demonstrates that Pkpc-1::GFP is expressed in the IL2s. The scale bar represents 5 mm. (D)
kpc-1(
gk8) defects in dauer-specific IL2Q arborization are rescued by constructs of either an IL2-specific promoter driving full-length wild-type
kpc-1 (Pklp-6::KPC-1) or full-length
kpc-1 tagged with dsRed and driven by the
kpc-1 endogenous promoter (KPC-1::dsRed). Rescue was assessed by examination of dauer-specific IL2Q arborization in
kpc-1(
gk8) dauers expressing Pklp-6::GFP in three independent transgenic lines (n = 22-25 dauers per line). A mean 6 SEM of the three lines is given. Statistical tests comparing the rescued lines with the WT and
kpc-1(
gk8) cannot be performed due to a lack of variation in controls; however, Pklp-6::KPC-1 and KPC-1::dsRed are not statistically different (Fisher's exact test, p = 0.9303). (E) z projection of
kpc-1(
gk8) dauer head expressing KPC-1::dsRed. In most neurons, KPC-1::dsRed is localized exclusively within the cell bodies and is not observed in neuronal processes. However, in the ventral nerve cord KPC-1::dsRed is found in both cell bodies and neuronal processes (see Figure S6B). The scale bar represents 10 um.