Fig. 2 Worm sleep deprivation triggers the UPRER. a Example of pre- (top) and post- (bottom) deprivation fluorescence of the
hsp-4p::gfp reporter. Priorto deprivation the reporter was prominently observed in the seam cells. b Fluorescence of the
hsp-4p::gfp reporter before and after deprivation, mockdeprivation, and stimulation of mid-L4 larvae. c Quantification of
hsp-4 expression using real-time PCR. Error bars depict 99% confidenceintervals (1 biological replicate, 20 animals per sample). d Fluorescence of the
hsp-4p::gfp reporter in mutants where the function of theUPRER genes
ire-1 and
xbp-1 was lost. On these mutant backgrounds sleep deprivation did not upregulate the expression of
hsp-4. In allbox plots, horizontal lines, boxes, and bars depict medians, 1st and 3rd quartiles, and 5th and 95th percentiles, respectively. Crosses denoteoutliers. Single and double asterisks denote significant differences with p < 0.05 and p < 0.01, respectively