Figure 2.
ceh-13 expression. (A-D) Four different focal planes of a comma-stage embryo stained with the anti-CEH-13 antibody; (E-H) corresponding DAPI stainings. Strong CEH-13 staining is detected in the lateral hypodermal cells H2 and V1 and in cells of the prospective ventral nerve cord (asterisks, VNC). CEH-13 expression in anterior dorsal hypodermal cells (arrowheads, hyp) and in anterior body wall muscle cells is indicated. Expression is also found in some unidentified cells located in the anterior part of the embryo. (I) CEH-13 distribution in a 15-16 hour L1 larva; (J) corresponding DAPI staining. V1 to V6 are lateral hypodermal cells, Vx/Vx.x are descendants of these cells; a, anterior; p, posterior (left view). V1.pxx cells are not fluorescing in the L1 larva. In contrast, all V1 to V4 descendants express
ceh-13 in L2 larvae (data not shown). (K-M) Embryonic expression pattern of the C. elegans Hox genes. Immunostainings of CEH-13 (K) and LIN-39::LACZ (L) in 1.5-fold embryos. Arrows indicate the borders of the LIN-39::LACZ expression domain. (M) Schematic drawing showing the expression domains of the different C. elegans Hox genes (adapted from Wang et al., 1993). Anterior, left. Bar, 10 µm.