Figure 4. Tissue-specific localization of
dpy-31. Expression of GFP reporter driven by the
dpy-31 promoter is detected in the hypodermal cells. (A) Fully elongated embryo and (B) L2 larva carrying a multicopy array of pD31P3.4G, which contains the
dpy-31 promoter sequence fused to GFP with a NLS. (C and C') L4 larva carrying a multicopy array of pD31P3.4G-N, which is a NLS negative version of pD31P3.4G. No expression of DPY-31 is detected in the seam cells (open arrowheads), which have not yet undergone fusion in this animal. The rectal epithelial cells expressing DPY-31 are indicated by an open arrow. Bars: A, 10 um; B, 20 um; C and C', 50 um.