Figure 3. CSR-1, DRH-3, EKL-1, and EGO-1 Are Expressed in the Germline. (A) Western blots of developmentally staged protein lysates (left) or various germline mutant lysates (right) probed for EGO-1, DRH-3, EKL-1, CSR-1 (multiple isoforms), and tubulin (as a loading control). L1, L2, L3, and L4 are larval stages; YA, young adults; GA, gravid adults; Embryos, mixed stage embryos. GA 25 C, gravid adults grown at 25 C;
fem-1(
hc17), no sperm at 25 C;
fog-2(
q71), enriched to 95% males by filtration (20 C); and
glp-4(
bn2), no germline at 25 C.(B) Wild-type perinuclear germline localization of DRH-3, CSR-1, and EGO-1 (left, yellow) (DNA, center, blue).(C) DRH-3 (left, green) colocalizes with the P granule component, PGL-1 (center, red; DNA, blue).(D) DRH-3 and CSR-1 (left, yellow) remain localized to P granules in the embryonic P cell lineage (dashed circles; DNA, center, blue).(E) Single confocal sections of PGL-1 (red) in wild-type and
csr-1(
tm892) mutant germlines through the germline surface and core. P granules become detached from the nuclear periphery in
csr-1(
tm892) (DNA, green; distal is to the left).