Figure 1. Developmental pattern of EGO-1 expression. (A) An 179-kDa protein is detected in extracts from wild-type (N2) adults but absent in extracts from
ego-1(
om97) adults. The 179-kDa band was also not detected in
ego-1(
om84) adults.
ego-1(
om97) has a premature stop codon and
ego-1(
om84) has a deletion; each mutation is predicted to encode a truncated protein (Smardon et al. 2000). (B) Myosin was used as a positive control. (C) EGO-1 protein is barely detectable in late L2/L3 larvae (L3). The level is higher in L4 larvae (L4) and in adults (A). This pattern mirrors the mRNA expression pattern. (D) Myosin was detected at approximately equivalent levels in each sample. See text.