Figure 1. CDKL-1 acts independently of DYF-5 to regulate morphology of simple but not complex cilia in C elegans.:A,B) Representative images of ASH cilia (A) and quantification of ASH cilia lengths (B) in the indicated genetic backgrounds. ASH cilia were visualized via expression of
sra-6p::myr-gfp. Arrowheads indicate cilia base; arrows indicate dendrite. Anterior is at top. Scale bar: 4 μm.C,D) Representative images of AWA cilia (C) and quantification of AWA cilia morphologies (D) in the indicated genetic backgrounds. AWA cilia were visualized via expression of
gpa-4pΔ6p::myr-gfp. Arrowheads indicate cilia base; arrows indicate dendrite; asterisk indicates dendritic branches. Anterior is at top left. Scale bar: 10 μm.Alleles used were:
cdkl-1(
ok2694) and
dyf-18(
ok200). Images shown are from adult hermaphrodites. *** and ###: different between indicated at P < 0.001 (one way ANOVA with Bonferroni posthoc test (B), Kruskal-Wallis with Bonferroni posthoc test (D). ns - not significant. n ≥30 neurons each.E) In neurons containing rod-like cilia, DYF-18 acts upstream of both DYF-5 and CDKL-1; DYF-5 and CDKL-1 act independently to inhibit cilia length. In the AWA neurons, DYF-18 also acts upstream of DYF-5 to regulate their complex cilia architecture. CDKL-1 appears to play only a minor role in shaping AWA cilia. XBX-4 regulates DYF-18 in multiple sensory neuron types (Maurya and Sengupta, 2021).