Figure 2. SNAP-25 is expressed in the neurons. Whole mount immunostaining was performed with antibodies specific for SNAP-25 or synaptotagmin. A and C, SNAP-25 expression in a wild-type hermaphrodite head (A) and a male tail region C. B and D, Synaptotagmin expression in the same animals as in A and C, respectively. SNAP-25 was expressed exclusively in the neurons, unlike synaptotagmin, which is expressed also in other secretory cells. The scale bars represent 100 um. E-G, SNAP-25 and synaptotagmin are colocalized in the wild-type animal. E, SNAP-25 expression on the ventral cord of an adult animal; F, synaptotagmin expression of the same animal as in E; and G, the merged image of E and F. The scale bar represents 10 um. H and I, SNAP-25 is not mislocalized in the unc-104
mutant background. H, SNAP-25 expression in an unc-104
mutant. I, Synaptotagmin expression in the same animal as in H. While synaptotagmin staining is abolished in the dorsal cord (arrowheads in I), SNAP-25 is still normally located on the dorsal cord (arrowheads in H), indicating that SNAP-25 is on the plasma membrane. J, SNAP-25 expression in a C. briggsae animal. K, Synaptotagmin expression in the same animal as in J. SNAP-25 is expressed in neurons in C. briggsae. The scale bars represent 100 um.