(E-J) SYGL-1 and LST-1 proteins in dissected adult gonads. (E-H) Representative sliceor (I-J) maximum intensity z-projections of distal gonad stained with alpha-FLAG (SYGL-1, magenta), alpha-HA (LST-1, yellow), alpha-GLD-1(green), and DAPI (cyan). Dashed line, gonadal outline; asterisk, distal end. Scale bar is 20 um in all images, except 5 um in (E) and(G) insets. (E)
sygl-1(
q828); qSi49[Psygl-1::3xFLAG::
sygl-1::
sygl-1 3'end]. (F)
sygl-1(
q828). (G)
lst-1(
ok814); qSi22[Plst-1::
lst1::1xHA::
lst-1 3'end]. (H)
lst-1(
ok814). See S1A-S1C Fig for whole gonad images.