Figure 1. New alleles of
phf-10 affect development and behavioral responses to ethanol:A. The
phf-10 locus produces at least three isoforms from two promoters. An alternatively spliced exon is indicated in grey and PHD domains are indicated by orange bars. Two new alleles of
phf-10,
rd21 and
rd22, were created by CRISPR/Cas9 genome editing using guide RNAs targeting the first and fifth exons; V indicates cut sites. B. Effects of
phf-10 alleles on development. Homozygous
phf-10 mutants were derived from the indicated parental genotype; n = number of homozygous mutant animals scored.
phf-10(
rd22) mutants had a low percentage of zygotic gonadogenesis defects and completely penetrant maternal effect embryonic and larval lethality.
phf-10(
rd21) mutants had very little lethality and no gonadogenesis defects. NA, not assessed. C. Acute behavioral responses to alcohol. Wild type worms (N2) develop AFT to ethanol, as indicated by a statistically significant increase in relative speed between 10 and 30 minutes of exposure. The degree of AFT is quantitated on the right Y-axis. Two loss-of-function alleles of
phf-10 have reduced AFT to ethanol.
phf-10(
rd21) mutants develop AFT (left), but the degree of AFT is significantly less than N2 (right).
phf-10(
rd22) mutants do not develop AFT. Paired two-tailed Student's t tests were used for statistical comparisons; **p ≤ 0.01, *p ≤ 0.05.