Figure 8. Mutations in the
che-1 locus eliminate adoption of the ASE(L/R) fate (class I phenotype). (A) ASE(L/R) expression of
gcy-7::gfp (otIs3),
lim-6::gfp (otIs114),
gcy-5::gfp (ntIs1), and
flp-6::gfp (otIs125) in adult wild-type and adult
che-1 (
ot27) mutant animals. ASE-specific expression of all reporters is lost in
che-1 mutants. Arrows in the "
lim-6::gfp" panel denote the excretory gland cell that expresses
lim-6::gfp and the ADF or AFD neuron class in the
flp-6::gfp panel; note that gfp expression in neither of these cell types is affected in
che-1 mutants. Asterisks denote gut autofluorescence. (B) Quantification of
che-1 effects on ASE gene expression patterns. (C) Schematic CHE-1 protein structure (drawn to scale), denoting the position of mutant alleles retrieved from our screen. The deletion breakpoints in
ot66 have not been sequenced but are inferred by PCR.