(C) Time lapse images of TSP-15::GFP [51] expression patterns and
tsp-15 null embryos during embryogenesis. Upper panels are confocal images of
tsp-15::gfp expression and Nomarski images of the corresponding embryo. Developmental stages are indicated on the top of the micrographs.
tsp-15::gfp expression was visible in quadrants of cells along the anteroposterior axis. The expression was decreased and body surface expression was visible around the three-fold stage. TSP-15::GFP expression is prominent in lateral hypodermal cells. Lower panels show representative images of
tsp-15(
ok854) null mutant embryos. Each developmental stage is the same as shown in the upper panels. The
tsp-15(
ok854) embryo developed normally, however, at terminal embryogenesis,
tsp-15(
ok854) embryos shrunk and failed to maintain the vermiform shape. Inset represents a different focal plane, which depicts an abnormal body protrusion in
tsp-15 null embryos. Scale bars indicate 10 um.