Figure 2. Clathrin and AP2 likely act in the same genetic pathway as CED-1 and CED-6. (A and B) Quantification of germ cell corpses in
ced-1(
e1735) and
ced-6(
n2095) (A) or
ced-2(
n1994) and
ced-5(
n1812) (B) mutants treated with Ctrl RNAi and RNAi of
chc-1,
dpy-23 and
apb-1. Cell corpses were scored in animals at 24 and 36 h after the L4 molt. Error bars represent SEM. Comparisons were made between control RNAi and RNAi of
chc-1,
dpy-23 and
apb-1 using unpaired t-tests. ** p,0.001; all other points had p.0.05. (C) Representative images of cell corpse labeling by APA-2::GFP in N2,
ced-1(
e1735),
chc-1(RNAi) and
ced-6(RNAi) germ lines. (D) Representative images of cell corpse labeling by mCherry::CHC-1 in N2, apb- 1(RNAi),
ced-1(RNAi) and
ced-6(RNAi) germ lines. In (C) and (D) arrows indicate cell corpses labeled by APA-2::GFP or mCherry::CHC-1 while arrowheads indicate unstained corpses. Bars, 10 um.