Figure 4.
osm-11 Is Expressed in VPCs and Other Tissues (A) OSM-11 expression in seam cells of L1 larvae detected using anti-OSM-11 antisera. The seam cells on the right side of an L1 animal are in focus; the seam cells on the left side are visible and slightly out of focus. OSM-11 was not expressed in seam cells or hypoderm at other larval stages. (B) OSM-11 expression in the developing uterus of L4 larvae. Left, anti-OSM-11 antisera staining; right, visible light image. (C) OSM-11 expression in vulval precursor cells (VPCs; arrowheads) in L3 larvae. The top panels show anti-OSM-11 antisera staining of VPCs prior (top left) and immediately after (top right) cell fate specification as assessed by
lip-1p::gfp expression. An overlay of anti-OSM-11 staining and
lip-1p::gfp expression shows that OSM-11 is concentrated on the apical surface of the VPCs (bottom right); this was confirmed using an
ajm-1::gfp fusion (unpublished data). (D) OSM-11 expression in seam cells and spermatheca in adult animals. An
osm-11p::gfp reporter gene containing
unc-54 3' UTR sequences is expressed in adult seam cells (left); anti-OSM-11 antisera was used to confirm seam cell and spermatheca expression (right). No OSM-11 was detected in neurons of larvae or adult animals (unpublished data); embryonic expression was not characterized. In (A-D), the scale bar represents 10 um.