Figure 1.
cdc-7(
tm4391) but not
cdc-7(
knu709) is temperature-sensitive sterile. (A) Schematic of
cdc-7 gene locus and location of
tm4391 and
knu709 deletions.
knu709 deletes 2,284 nucleotides, beginning 163 nucleotides upstream of the
cdc-7 ATG start and ending 136 nucleotides downstream of the
cdc-7 TAA stop, within the
cdc-7 3'UTR. This deletion does not impact upstream or downstream genes. (B)
cdc-7(
tm4391) worms are temperature sensitive sterile, with higher temperatures being progressively worse for fecundity. At 25 degC, 90% of individual worms tested were sterile (Total n= 48. Number of plates with progeny = 3, number without progeny = 45). There was an intermediate effect at 20 degC with 56% sterility (Total n= 49. Number of plates with progeny = 21, number without progeny = 28).
cdc-7(
tm4391) was most fertile when kept at 16 degC, with only 30% sterility (Total n= 49. Number of plates with progeny = 35, number without progeny = 14). N2 controls had no observed sterility at any temperatures (for 25 degC, 20 degC and 16 degC respectively, total n= 50, 50, 50. Number of plates with progeny = 50, 50, 50). (C)
cdc-7(
knu709) were substantially less likely to be infertile and infertility did not correspond to temperature (for 25 degC, 20 degC and 16degC respectively, percent sterile = 0%, 10%, 4%. Total n= 50, 49, 45. Number of plates with progeny = 48, 44, 43. Number of plates without progeny = 0, 5, 2).