Figure 2. Expression patterns for
bus-2. (A-C) Expression from the rescuing operon construct
bus-2::GFP in a
bus-2(
e2687) background, strain CB6616. (D and E) Expression from the rescuing operon construct
bus-2::mCherry in a
bus-2(
e2687) background and corresponding mCherry construct, strain CB6846. (A) Whole adult worm, showing seam-cell expression. (B) Detail of oviduct, showing strong spermathecal expression. (C) Detail of tail after exposure to M. nematophilum, illustrating tail-swelling (arrowed) to demonstrate functional rescue of
bus-2 activity; seam-cell expression is visible (white arrowheads). (D) Adult posterior region, showing intestinal expression. (E) Adult head region, showing head muscle expression. Scale bars: 25 um.