Figure 2. Expression patterns for bus-2
. (A-C) Expression from the rescuing operon construct bus-2
::GFP in a bus-2
) background, strain CB6616. (D and E) Expression from the rescuing operon construct bus-2
::mCherry in a bus-2
) background and corresponding mCherry construct, strain CB6846. (A) Whole adult worm, showing seam-cell expression. (B) Detail of oviduct, showing strong spermathecal expression. (C) Detail of tail after exposure to M. nematophilum, illustrating tail-swelling (arrowed) to demonstrate functional rescue of bus-2
activity; seam-cell expression is visible (white arrowheads). (D) Adult posterior region, showing intestinal expression. (E) Adult head region, showing head muscle expression. Scale bars: 25 um.