Figure 2.
ife-2 is expressed in both somatic and germline tissues. (A-F)
ife-2 expression in somatic cells as detected by IFE-2::GFP fluorescence in a blastula (A,D) and comma staged (B,E) embryo, larval stage L2 (C) and adult hermaphrodite (F). For embryos, both dark-field fluorescence (A,B) and DIC images (D,E) of the same individuals are shown. Full field imaging of adult hermaphrodite fluorescence (F) is shown at one-tenth the magnification of A-E. (G)
ife-2 expression, as detected by IHC with anti-IFE-2 antibodies, is observed in wild-type germline cells (left panels) but not in those of
ife-2(
ok306) mutants (right panels). DT, distal tips. Scale bar: 20 um (A,B,D,E), 50 um (C,F) and 10 um (G).