FIG. 3. Graphic representation of post-embryonic mRNA abundance of
pdi-1 and
cyp-9. Semiquantitative RT-PCR was performed simultaneously with primers corresponding to the genes
cyp-9,
pdi-1, and
ama-1 on mRNA samples corresponding to 2hr time periods between the first larval and adult stages from synchronous cultures. Products were electrophoresed, blotted, and hybridized with gene-specific probes. The corresponding gene-specific bands were excised and counted by scintillation, and the signal of the test genes were expressed as ratios against the signal of the control gene
ama-1. The x axis refers to time in hours in post-embryonic development; L1, L2, L3, and L4 refer to the corresponding larval stages from the nematode life cycle. L1, Larva 1. The y axis is the ratio of the detected test gene mRNA over control
ama-1 mRNA.