Figure 3. Expression of GFP-reporter constructs. (A) K08B12.1 was predicted to express in hypodermis; the reporter construct expressed exclusively in hypodermis. Expression was variable, strongest in embryo-L1, though detectable in all stages. (B) F58H1.2 was predicted to express in hypodermis; the reporter construct expressed exclusively in hypodermis. Expression was variable, strongest in embryo-L1 and not detectable in adults. (C) F55H12.4 was predicted to express in the hypodermis. pF55H12.4::GFP expressed in hypodermis, vulva, anus, and to a lesser extent pharynx. Hypodermal expression was highly variable and was strongest in L4 and Adult stages. (E) C29F5.1 was predicted to express in muscle. The reporter construct was observed in body wall, vulval, and anal but not pharyngeal muscle in all stages. (F) F13D12.6 was predicted to express in the intestine and the reporter construct expressed exclusively in intestinal cells at all stages. (D)
gnrr-1 was predicted to express in neurons. Strong expression of pgnrr-1::GFP was seen in various head neurons at all stages. Expression was also observed in the anterior pharynx and ventral nerve cord neurons. (A,B) Seam cell exclusion is observed in these lines, which is typical of hypodermally expressed genes; see Gilleard et al. [77] for examples of hypodermal expression.