Figure 7. ACR-7 acted in the pharyngeal muscle. (A) ACR-7 was highly expressed in the pharynx as indicated by the arrows. A, anterior; P, posterior; D, dorsal; V, ventral. Scattered expression was also observed in the tail and vulval regions. (B) Clozapine inhibited pharyngeal pumping in wild-type animals in a concentration-dependent manner. Suppression of clozapine-induced inhibition of pharyngeal pumping was seen in acr- 7(tm863
) animals, and rescue was observed with expression of ACR-7 in the mutant background. A putative acr-7
promoter was utilized to drive expression for the acr-7
) mchEx40 strain, whereas the pharyngeal muscle-specific myo-2
promoter was utilized for the acr-7
) mchEx207 strain. (C) Both nicotine and clozapine inhibited the pumping rate in N2 animals. d-TC partially blocked these effects. Importantly, d-TC alone had no effect on pumping. (D) d-TC failed to block clozapine- or nicotine-induced inhibition of pumping in acr-7
(lf) animals. * P,0.05; ** P,0.01; *** P,0.0001.