Fig 3. Defective membrane trafficking of hTAC::GFP and GLUT1::GFP in
sgk-1(null) mutants. Confocalimages of wild type (A, A', G),
sgk-1(
ok538) (B, B', H) and
sgk-1(
mg455) (C, C') intestinal cells expressing hTAC::GFP or GLUT1::GFP. The apical, basal and lateral membranes are indicated by open arrow heads, solid arrow heads and arrows, respectively. Scale bars: 5 um. See Fig 2 legend for explanations of different focal planes. (D-F) Quantitation of average pixel intensity of hTAC::GFP localized in the cytoplasm (D), on the basal membrane (E) or lateral membrane (F). The average intensity is denoted with a red line. *** P value <0.001, ** P value <0.01 (Wilcoxon rank sum test).