Figure 5. Sub-cellular localization patterns for some category 5, 6 and 7 GFP-tagged proteins. All 5 panels show single symmetrical, spindle-shaped body wall muscle cells exhibiting GFP-tagged protein localization. The category 5 protein, T03G6.3, (A), is most likely a component of the sarcoplasmic reticulum. The category 6 protein, T22A4.3, (B), is found in the dense bodies, thick filaments and/or M-lines and the ER/SR. The category 7 proteins, Y71F9B.3 (C), ZK637.3 (D) and R07E5.7 (E), all localize to the muscle cell membrane in various patterns. Bars represent 10 um.
(F) Immunofluorescence image of a wild-type adult with VHA-5 antiserum; VHA-5 forms dots in the epidermis. (G) Immunogold labeling against VHA-5 (gold beads, arrowheads); VHA-5 localizes mainly to apical membrane stacks of the epidermis.