Figure 5. try-5
is expressed in the male somatic gonad. Confocal and transmitted-light (TL) images of transgenic males bearing try-5
reporter insertions. In TL images, boundaries of regions of the somatic gonad are labeled as sv, seminal vesicle; vl, valve; and vas, vas deferens. Scale bars, 10 mm. (A) jnSi49[Ptry-5::GFP::H2B]; try-5
) L4 male. In focal planes shown, GFP is visible in the four GFP-positive seminal vesicle cells, two of four GFP-positive valve cells, and six of twelve GFP-positive cuboidal cells. (B) jnSi62[Ptry-5::TRY-5::GFP]; try-5
) L4 male. TRY-5::GFP is concentrated at the apical side of seminal vesicle, valve and cuboidal cells. (C) jnSi62[Ptry-5::TRY-5::GFP]; try-5
) 48 hr adult male. Both large and small TRY-5::GFP globules are present in the cuboidal cells and valve region. In the seminal vesicle, TRY-5::GFP is present in the proximal region near the valve. (D) jnSi62[Ptry-5::TRY-5::GFP]; swm-1
) 48 hr adult male. TRY-5::GFP has expanded into the seminal vesicle lumen and sperm are activated. (C9, D9) Images of seminal vesicle regions outlined in C and D with intensity levels optimized for the fainter GFP signal in these tissues. Arrows indicate GFP in the distal sheath-like cells of the seminal vesicle. (E) Paired DIC, epifluorescence and merged images of a jnSi62[Ptry- 5::TRY-5::GFP]; try-5
) 72 hr adult male in the region of the seminal vesicle. Localization of TRY-5::GFP is correlated with activated sperm (also see Table S4 and S5).