Figure 6. Subcellular localization of PST-1 and PST-2. (A) Schematic of C-terminal fusions of PST-1A, PST-2 and of a mannosidase II fragment (MANS) that targets expression to the Golgi (Rolls et al., 2002) with the fluorescent proteins are indicated. All constructs are under control of the pst-1a
promoter (supplementary material Fig. S7). Not drawn to scale. (B) PST-1A::YFP and PST-2::CFP display partial colocalization. Magnification, 630x. (C) The Golgi marker MANS::RFP displays partial colocalization with both PST-1A::YFP and PST-2::CFP. (D) Triple stain of PST-1A::YFP, PST-2::CFP and the MANS::RFP Golgi marker. Arrowheads denote lack of colocalization in an intestinal cell in all cases. All insets created with 2x digital zoom. Magnification, 630x.