Figure S4. Tissue-expression pattern of SFT-4. (A) SFT-4 is highly expressed in the intestine, body wall muscle, and spermatheca. GFP-SFT-4 expressedunder the control of the sft-4
promoter (Psft-4-GFP-SFT-4) was detected in the intestine (left; arrowhead), body wall muscle (middle; arrowheads), andspermatheca (right; arrowhead). Dotted lines indicate the outlines of intestines, and lines indicate the outlines of the body wall. Bars, 10 um. (B) An anti-SFT-4 antibody recognizes endogenous SFT-4 proteins. Immunostaining using an anti-SFT-4 antibody mainly labeled the ER reticular network and punctatestructures in intestinal cells in control animals (left) but not in sft-4
(RNAi) animals (right). Dotted lines indicate the outlines of intestines. Bars, 10 um.