Figure 4.
lin-23 developmental mRNA expression. (A) Northern blot of total RNA probed initially for
lin-23 mRNA (upper) and then 18S rRNA (lower). Relative intensities of the
lin-23 signals, using rRNA to correct for loading differences, were 96% (E, mixed embryos), 34% (L1 larvae), 33% (L2), 27% (L3), 33% (L4), 44% (yA, young adults), and 100% (grA, gravid adults). (B) In situ hybridization of adult wild-type hermaphrodites with control
lin-23 sense RNA (top) or antisense RNA (bottom). Note that eggs inside hermaphrodites are generally not permeable to RNA probes. (C) Wild-type zygote hybridized with
lin-23 sense RNA (top) and wild-type zygote and 60-cell stage embryo hybridized with
lin-23 antisense RNA (bottom).