Figure 1. Comparison of genome editing efficiency between wild type Cas9 and SuperFi-Cas9 in C elegans:(A) Representative images of wild type and different mutant worms. Worms were grown under identical conditions and the images were taken at young adult stage for all the strains. Scale bar = 100 µm. (B) Efficiencies of Cas9- and SuperFi-Cas9-mediated mutagenesis through non-homologous end joining. Note that in F1 worms harboring the wild type Cas9, the mutagenesis rates for
unc-9 and
phm-2 were much higher than that for
dpy-11, which was likely due to the presence of a GG motif at the 3' end of the protospacer sequences for
unc-9 and
phm-2 but not for
dpy-11 (see methods). This observation is consistent with a previous report showing that a GG motif at the 3' end of the protospacer sequence dramatically enhances genome editing by CRISPR/Cas9 (Farboud and Meyer, 2015). (C) Efficiencies of Cas9- and SuperFi-Cas9-mediated mutagenesis through homology-directed repair. Mutagenesis rate = (number of F1s yielding mutants/number of transgenic F1s) x 100.