Figure 1: Signal intensity and sperm parameters in the UX993 strain. (A) Images and brightness of spermatid nuclei from UX993 and its parent strains. (B) Single perspective of a 3-D rendering generated from a z stack taken through the gonad. The brightfield image is overlaid with fluorescence. When imaged in vivo, UX993 sperm nuclei (red) are clearly visible, and the sperm populations in the oviduct, spermatheca, and uterus are easily distinguishable from one another. Female germline and oocyte nuclei are pseudocolored white. The cluster of sperm within the spermatheca is indicated by the white dotted line. (C) UX993 hermaphrodite fertility is comparable to that of wild-type hermaphrodites. Bar graphs show average progeny counts excluding those from the first 24 hours post-L4; error bars indicate 95% confidence intervals. ***, p<0.01; ns, not significant (Student's t test). (D) N2 and UX993 hermaphrodites have indistinguishable numbers of sperm. Bar graph shows average sperm counts in 24-hour post-L4 hermaphrodites; error bars indicate 95% confidence intervals. n=15-16. (E) UX993 hermaphrodite sperm have wild-type usage patterns: they are outcompeted by wild-type male sperm, and they outcompete competition-deficient
comp-1 male sperm. Each dot represents one mating, and results for one representative repeat are shown. Gray lines indicate median values. **, p<0.001 (Kolmogorov-Smirnov test). Scale bars, 20 um.