Figure 1. Expression of
ceh-13 in C. elegans early embryos. (A-D) 28-cell-stage embryo (this embryo
has16 AB-derived cells, we call it a 16-AB stage). (A,C) Ventral and (B,D) dorsal views. (A,B) Immunofluorescent staining of Ep and ABxxxp cells with anti-CEH-13 antibodies. The ABarap cell stains very weakly. (C,D) The same embryo stained with the DNA-binding dye DAPI. The names of the blastomeres are indicated (see Materials and Methods for blastomere identification); lower cases are used for AB-derivatives. (E) 32-AB-stage embryo expressing the
ceh-13::gfp transgene. All fluorescent nuclei are visible. CEH-13::GFP is present in Epl and Epr (arrowheads) and in 14 out of 16 daughters of ABxxxp (arrows). The image has been generated by adding fluorescent pictures of two different focal planes. (F) Confocal microscope section of an about 50-cell-stage embryo showing CEH-13::GFP in Da and Dp (arrows). All embryos are oriented with the anterior end to the left. Embryos measure approximately 50 µm along their anteroposterior axis.