Figure 9. Expression of an
nlp-36p::gfp fusion gene is entrained by temperature cycles. (A) gfp expression in transgenic animals carrying stably integrated copies of an
nlp-36p::gfp fusion gene during temperature entrainment (CW) and free-running (WW) conditions. Adult animals were examined under 100 magnification. (B) Quantification of intestinal
nlp-36p::gfp fluorescence intensity (in arbitrary units [A.U.]) at the indicated time points during entrainment or free-running days. Wild-type transgenic animals were entrained to either WC/CC (gray bars) or CW/WW (red bars) cycles. Error bars indicate the s.e.m. Data shown are from two independent experiments with 15-20 animals at each time point. (C)
nlp-36p::gfp-expressing transgenic animals were subjected to CW entrainment cycles and moved to WW conditions. After 6 h at WW, a subset of animals were subjected to a pulse of 15 C temperature for 6 h and returned to WW conditions. Intestinal fluorescence intensities in animals subjected or not subjected to the cold pulse are indicated in blue and gray, respectively. Error bars indicate the standard deviation. n = 15-20 animals each at each time point.