Figure 1. MIG-10 does not contribute significantly to the process of dorsal intercalation: Figure 1. MIG-10 is not essential for the process of dorsal intercalation. (A) Schematic showing the CRISPR-based tagging strategy. All isoforms of MIG-10 include the N-terminal exon to which mNeonGreen (green) was fused. (B-C) MIG-10::mNeonGreen expression shows that MIG-10 isoforms are not appreciably expressed in the dorsal epidermis. (B) MIG-10::mNeonGreen expression from a dorsal view during the process of the dorsal intercalation. (C) MIG-10::mNeonGreen expression from a ventral view before ventral enclosure, (Ca) at the beginning of ventral enclosure, (Cb) during mid ventral enclosure, and (Cc) during early elongation. Arrows indicate muscle quadrants. Images are maximum intensity Z projections of 16 focal planes. Scale bars = 10 μm. (D) DIC images of N2 and
mig-10(
ct41) embryos at early, middle and late stages of dorsal intercalation. Posterior cells are pseudocolored for clarity. (E) Confocal image of MIG-10::mNeonGreen with epidermally expressed LifeAct::mCherry. Dotted lines indicate sites of reslicing in Z. Vertical (Ea, Eb, Ec) and horizontal (Ed, Ee, Ef) reslices depicting merged (Ea, Ed), LifeAct::mCherry (Eb, Ee), and MIG-10::mNeonGreen (Ec, Ef) are also shown. Scale bar = 10 μm.