Figure 1. ZOO-1 Junctional Recruitment Is Dependent on HMR-1/E-Cadherin and VAB-9/Claudin, but Not on HMP-2/b-Catenin or HMP-1/a-Catenin.(A-L) Confocal images of elongating embryos stained for ZOO-1 (green in [A], [D], [G], [J], [M]), AJM-1 as a junctional marker (red in [B], [E], [H], [K], [N]), and the merged image (C, F, I, L, O). Wild-type (A-C), dlg-1
(RNAi) (D-F), and hmp-1
(RNAi) (G-I) embryos display proper junctional localization of ZOO-1, despite disruption of AJM-1 localization in the case of dlg-1
(RNAi) (E). (J-L) hmr-1
(RNAi) embryo exhibits abrogated junctional ZOO-1 staining, though staining persists in sarcomeres (J, arrow).(M-O) vab-9
) embryo lacks junctional ZOO-1 staining, though ZOO-1 localization in sarcomeres in unaffected ([M], arrow).Scale bar represents 10 μm.