Fig 6. Depletion of
gon-1 causes DAF-7 secretion defects. (a, b) Fluorescent images of DAF-7::mCherry, and DIC images (inset) in the head neurons of a wild-type animal (a) and a
gon-1(
tm3146) mutant animal (b) (160 msec. exposure time). The scale bar indicates 10 um. (c) Quantification of the mCherry fluorescence in neurons. The graph represents the combined results of 3 independent experiments (n > 20 neurons per strain). *p < 0.05. Bars represent themean SE. (d, e) Representative fluorescent images of DAF-7::mCherry in the coelomocytes of wild-type (d) and a
gon-1(
tm3146) mutant (e) animals (100 msec. exposure time). Coelomocytes are outlined with dotted circles in the fluorescent images. Insets show DIC images. The scale bar indicates 10 um. (f) Quantification of the mCherry fluorescence in coelomocytes. The graph represents the combined results of 3 independent experiments (n > 30 coelomocytesper strain). Fluorescence intensity was examined using ImageJ (NIH, Bethesda, MD). *p < 0.05. Bars represent the mean SE.IL is WBbt:0003888" Certain