Fig 3. FLCN-1 and RME-4 are the candidate GEFs and TBC-10 is a candidate GAP for RAB-35. (A and B) Bar graph displaying results ofthe epistasis analysis between
rab-35 and genes that encode candidate GEF (A) and GAP (B) proteins for RAB-35. The mean numbers of cellcorpses in 1.5-fold stage wild-type and various single and double mutant strains are presented. For each data point, at least 15 animals werescored. Error bars indicate sd. Brackets above the bars indicate the samples that are compared by the Student t-test. p-values are summarizedas such: , 0.001 < p < 0.05; , 0.00001 < p <0.001; , p <0.00001; ns, no significant difference. (C-E): Time-lapse recording of theengulfment of C3 and the beginning of phagosome maturation in the
tbc-10(
tm2790) mutant (C) and wild-type (D-E) embryos. Reportersare indicated on the side. "0 min" is the moment a nascent phagosome is just formed. Arrowheads indicate extending pseudopods. (C) Awhite arrow marks the nascent phagosome. Regions on the phagosomal membrane with enriched GFP::RAB-35 signal are marked by yellowarrows. (D) White and yellow arrows each mark the burst of RAB-35 signal on and the loss of FLCN-1 signal from the phagosome,respectively. (E) White and yellow arrows each mark the burst of RAB-35 signal on and the loss of TBC-10 from the phagosome,respectively.