Figure S4. Genetic Interactions between
nmr-1,
npr-5, and Serotonergic Signaling Mutants and Transgenic Rescue of Hyperactive Feeding in
nmr-1 Mutants, Related to Figure 5(A) Relative pharyngeal pumping rates of wild-type,
nmr-1 mutants, and
nmr-1 mutants bearing transgenes expressing an
nmr-1 cDNA under the control of either the
nmr-1,
tdc-1 or
cex-1 promoters. These transgenic animals were subjected to either
nkat-1(RNAi) or an RNAi vector control.(B) Relative pharyngeal pumping rates of the same strains used in (A) with ad libitum access to food, after 2 hr of fasting and 10 min of post-fast refeeding. In (A) and (B) ns: p > 0.5, ***p < 0.0001 ANOVA (Tukey).(C) Pharyngeal pumping rates of wild-type,
nkat-1,
aak-2, and
tph-1 mutants cultured on bacteria expressing either vector control RNAi or
nmr-1(RNAi). p < 0.0001 ANOVA (Sidak) comparing vector control treatment and
nmr-1(RNAi) treatment for each condition.(D) The pharyngeal rates of wild-type,
nmr-1,
flp-18, and
npr-5 mutants fed ad libitum, after a 2 hr fast, and 5, 30, 60 and 120 min after returning to food post-fast. **p < 0.005 ANOVA (Dunnett)(E) The pharyngeal pumping rates of wild-type,
aak-2,
npr-5 mutants, and
aak-2;
npr-5 double mutants. p < 0.0001 ANOVA (Tukey) comparing
aak-2 single and
aak-2;
npr-5 double mutants to both wild-type and
npr-5 mutants.In (A) through (E) error bars = 95% c.i., n = 10-14 animals per condition.